Signalling

Part:BBa_K2789009:Design

Designed by: Du liaoqi   Group: iGEM18_WHU-China   (2018-10-05)


optimized Ccas from BBa_K68102


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1310
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 715
    Illegal AgeI site found at 1720
    Illegal AgeI site found at 1915
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We have optimized the code in the Ccas and we deleted the EcoRI restriction site in the Ccas for future DNA ligation and digestion.


Source

Initial Ccas is from BBa_K68102

References

We have condon-optimeized Ccas BBa_K2789009 for E.coli from BBa_K608102 and deleted the EcoRI and SpeI site in the gene sequence. We paste the website of BBa_K608102 (https://parts.igem.org/Part:BBa_K608102:Design)